T-Type Calcium Channels

After extensive washing, the membranes were incubated using a horseradish peroxidase-conjugated goat anti-rabbit IgG (1: 20,000; Boster, Wuhan, China) at area heat range for 40?min

After extensive washing, the membranes were incubated using a horseradish peroxidase-conjugated goat anti-rabbit IgG (1: 20,000; Boster, Wuhan, China) at area heat range for 40?min.